Model fitted: LME or GEE model
log(hormones) ~ Study + GA at sampling + Study:GA at sampling LME only: Random intercepts for Study ID and Plate GEE only: Independence correlation structure
12.2.1 Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
PRL (ng/ml)
Chisq
Df
Pr(>Chisq)
(Intercept)
21.782244
1
<0.0001
***
Study
1.759986
1
0.2
ns
GA_samp
37.833421
1
<0.0001
***
Study:GA_samp
1.825160
1
0.2
ns
hPL (μg/ml)
Chisq
Df
Pr(>Chisq)
(Intercept)
107.37637
1
<0.0001
***
Study
63.37312
1
<0.0001
***
GA_samp
115.88145
1
<0.0001
***
Study:GA_samp
71.34390
1
<0.0001
***
GH2 (ng/ml)
Chisq
Df
Pr(>Chisq)
(Intercept)
9.974225
1
0.002
**
Study
26.572090
1
<0.0001
***
GA_samp
57.571753
1
<0.0001
***
Study:GA_samp
26.560416
1
<0.0001
***
Insulin (uU/ml)
model term
df1
df2
F.ratio
Chisq
p.value
1
Study
1
Inf
3.706
3.706
0.05
ns
3
GA_samp
1
Inf
0.183
0.183
0.7
ns
2
Study:GA_samp
1
Inf
2.665
2.665
0.1
ns
HOMA-IR
model term
df1
df2
F.ratio
Chisq
p.value
1
Study
1
Inf
4.810
4.810
0.03
*
3
GA_samp
1
Inf
0.432
0.432
0.5
ns
2
Study:GA_samp
1
Inf
2.900
2.900
0.09
ns
Glucose (mmol/L)
model term
df1
df2
F.ratio
Chisq
p.value
1
Study
1
Inf
0.138
0.138
0.7
ns
3
GA_samp
1
Inf
0.001
0.001
1.0
ns
2
Study:GA_samp
1
Inf
0.190
0.190
0.7
ns
12.2.2 Model summary
Ratio of Geometric means (95% CI)
PRL (ng/ml)
Var
Effect
P
(Intercept)
2.14 (1.24, 3.03)
<0.0001
StudySTOP
0.63 (-0.30, 1.56)
0.18
GA_samp
0.18 (0.12, 0.24)
<0.0001
StudySTOP:GA_samp
-0.04 (-0.10, 0.02)
0.18
hPL (μg/ml)
Var
Effect
P
(Intercept)
2.53 (2.04, 3.01)
<0.0001
StudySTOP
-2.02 (-2.52, -1.52)
<0.0001
GA_samp
0.17 (0.14, 0.20)
<0.0001
StudySTOP:GA_samp
0.14 (0.11, 0.18)
<0.0001
GH2 (ng/ml)
Var
Effect
P
(Intercept)
-1.04 (-1.70, -0.38)
0.002
StudySTOP
-1.77 (-2.45, -1.08)
<0.0001
GA_samp
0.16 (0.12, 0.20)
<0.0001
StudySTOP:GA_samp
0.12 (0.07, 0.16)
<0.0001
Insulin (uU/ml)
Var
Effect
P
(Intercept)
3.45 (2.16, 4.73)
<0.0001
StudySTOP
-1.31 (-2.64, 0.02)
0.05
GA_samp
-0.05 (-0.13, 0.04)
0.27
StudySTOP:GA_samp
0.07 (-0.01, 0.16)
0.10
HOMA-IR
Var
Effect
P
(Intercept)
2.16 (0.83, 3.50)
0.001
StudySTOP
-1.55 (-2.94, -0.16)
0.03
GA_samp
-0.06 (-0.14, 0.03)
0.21
StudySTOP:GA_samp
0.08 (-0.01, 0.17)
0.09
Glucose (mmol/L)
Var
Effect
P
(Intercept)
1.66 (1.30, 2.02)
<0.0001
StudySTOP
-0.07 (-0.43, 0.29)
0.71
GA_samp
0.00 (-0.02, 0.03)
0.84
StudySTOP:GA_samp
-0.01 (-0.03, 0.02)
0.66
12.2.3 Diagnostics
Model diagnositcs.
Diagnositcs for PRL (ng/ml)
Diagnositcs for hPL (μg/ml)
Diagnositcs for GH2 (ng/ml)
Diagnositcs for Insulin (uU/ml)
Diagnositcs for HOMA-IR
Diagnositcs for Glucose (mmol/L)
12.2.4 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
PRL (ng/ml)
Study
GA_samp
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
10
46.77984
8.557414
Inf
32.68503
66.95279
STOP
10
60.98198
1.984145
Inf
57.21453
64.99751
SCOPE
16
157.65554
4.671804
Inf
148.75981
167.08324
STOP
16
145.80292
10.047989
Inf
127.38133
166.88860
hPL (μg/ml)
Study
GA_samp
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
10
58.66173
5.1415039
Inf
49.40261
69.65621
STOP
10
37.37513
0.7441673
Inf
35.94469
38.86251
SCOPE
16
191.79504
2.4328863
Inf
187.08546
196.62318
STOP
16
239.75276
7.7025414
Inf
225.12153
255.33490
GH2 (ng/ml)
Study
GA_samp
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
10
1.5266388
0.1577893
Inf
1.246690
1.869452
STOP
10
0.9794978
0.0222874
Inf
0.936775
1.024169
SCOPE
16
4.6508967
0.0835049
Inf
4.490076
4.817477
STOP
16
5.2495097
0.2270129
Inf
4.822907
5.713847
Insulin (uU/L)
Study
GA_samp
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
10
19.77659
4.6279509
Inf
12.50145
31.28545
STOP
10
11.15166
0.3842101
Inf
10.42348
11.93070
SCOPE
16
14.96218
0.6124863
Inf
13.80862
16.21210
STOP
16
13.12747
0.9877112
Inf
11.32756
15.21337
HOMA-IR
Study
GA_samp
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
10
5.004074
1.2160703
Inf
3.107908
8.057111
STOP
10
2.360224
0.0858766
Inf
2.197770
2.534686
SCOPE
16
3.587401
0.1563751
Inf
3.293639
3.907364
STOP
16
2.735166
0.2179779
Inf
2.339631
3.197568
Glucose (mmol/L)
Study
GA_samp
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
10
5.395297
0.3344260
Inf
4.778085
6.092238
STOP
10
4.773670
0.0366162
Inf
4.702440
4.845979
SCOPE
16
5.475695
0.0719187
Inf
5.336536
5.618483
STOP
16
4.691147
0.0561450
Inf
4.582385
4.802490
12.2.5 Comparisons
Hormone
Contrast
GA_samp
Ratio of Geometric means (95% CI)
P-value
PRL (ng/ml)
STOP / SCOPE
10
1.30 (0.91, 1.88)
0.2
PRL (ng/ml)
STOP / SCOPE
16
0.92 (0.80, 1.07)
0.3
hPL (μg/ml)
STOP / SCOPE
10
0.64 (0.53, 0.76)
<0.0001
hPL (μg/ml)
STOP / SCOPE
16
1.25 (1.17, 1.34)
<0.0001
GH2 (ng/ml)
STOP / SCOPE
10
0.64 (0.52, 0.79)
<0.0001
GH2 (ng/ml)
STOP / SCOPE
16
1.13 (1.03, 1.24)
0.010
Insulin (uU/ml)
STOP / SCOPE
10
0.56 (0.35, 0.90)
0.02
Insulin (uU/ml)
STOP / SCOPE
16
0.88 (0.74, 1.04)
0.1
HOMA-IR
STOP / SCOPE
10
0.47 (0.29, 0.76)
0.002
HOMA-IR
STOP / SCOPE
16
0.76 (0.64, 0.91)
0.003
Glucose (mmol/L)
STOP / SCOPE
10
0.88 (0.78, 1.00)
0.05
Glucose (mmol/L)
STOP / SCOPE
16
0.86 (0.83, 0.89)
<0.0001
12.2.6 Barplots by Study
Barplots in this section may be misleading due to involvement of Study by GA interaction.